Abstract
Cervical cancer (CC) is the most common malignant tumor in gynecology, and metastasis is an important cause of patient death. MiRNAs (microRNAs) have been found to play key roles in cervical cancer metastasis, but the effect of miR-362-3p in CC is unclear. This study aimed to investigate the role of miR-362-3p in cervical cancer migration and invasion. We compared the expression levels of miR-362-3p in cervical cancer tissues and adjacent normal cervical tissues. In CC tissues, miR-362-3p expression was significantly down-regulated, which is related to the cancer stage and patient survival. MiR-362-3p can effectively inhibit the migration and invasion of cervical cancer cells. The dual-luciferase reporter assay results showed that BCAP31 (B cell receptor associated protein 31) is a direct target protein of miR-362-3p. The results of the immunohistochemical examination of clinical tissue samples showed that BCAP31 was abnormally highly expressed in cervical cancer, which was positively correlated with the clinical stage. BCAP31 knockdown exerted similar effects as miR-362-3p overexpression. Further GSEA analysis showed that BCAP31 may participate in multiple biological processes, such as protein transport, metabolism, and organelle organization. Our results suggest that miR-362-3p inhibits migration and invasion via directly targeting BCAP31 in cervical cancer, and restoring miR-362-3p levels may be a new treatment strategy for cervical cancer in the future.
Highlights
Cervical cancer (CC) is one of the common malignant tumors in women’s reproductive systems and the third leading cause of cancer mortality among females (Torre et al, 2015; Small et al, 2017)
The results showed that miR-362-3p expression in cervical cancer was about 35.7% of normal tissues (Figure 1A)
Recent research shows that more than 500,000 women are diagnosed with cervical cancer each year, 85% of which occur in low-middle developed areas, and the disease causes more than 300,000 deaths worldwide (Vaccarella et al, 2013; Cohen et al, 2019)
Summary
Cervical cancer (CC) is one of the common malignant tumors in women’s reproductive systems and the third leading cause of cancer mortality among females (Torre et al, 2015; Small et al, 2017). Research on the molecular mechanism that promotes CC metastases is of great significance. MicroRNA (miRNA) is a type of non-coding small RNA found in animals, plants and some viruses, which plays an important role in the post-transcriptional regulation of gene expression (O’Brien et al, 2018). Abnormal changes in miRNA expression have been reported in several human cancers and are related to tumorigenesis and development (Reddy, 2015; Peng and Croce, 2016). It has been reported that some miRNAs, such as miR-1246, miR-362-3p Suppresses CC Through BCAP31
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