MicroRNA-27a rs895819 is associated with obesity in HIV infected preeclamptic Black South African women on HAART

Niren Ray Maharaj,Prithiksha Ramkaran,Siddharthiya Pillay,Anil Amichund Chuturgoon

doi:10.1186/s12881-016-0353-8

Niren Ray Maharaj, Prithiksha Ramkaran + Show 2 more

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https://doi.org/10.1186/s12881-016-0353-8

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Journal: BMC Medical Genomics	Publication Date: Dec 1, 2016
Citations: 5	License type: cc-by

Affiliation: University of KwaZulu-Natal

Abstract

BackgroundPreeclampsia (PE) and HIV/AIDS present a major health challenge globally. South Africa has the highest disease burden of both HIV/AIDS and PE in the world. Despite extensive research, the pathophysiology of these conditions is not completely understood, however a genetic predisposition in women may affect susceptibility. MiRNA-27a regulates adipogenesis and glucose metabolism. A single nucleotide polymorphism (SNP) in miRNA-27a (rs895819T > C) has shown to have disparate effects in various populations. This study investigated the frequency of rs895819 in pregnant normotensive and preeclamptic Black South African (SA) women.MethodsEnrollment into the study included: normotensive (n = 95; 45 HIV+; 80 analysed for rs895819T > C, age range: 16–46 years) and PE patients (n = 98; 45 HIV+; 56 analysed for rs895819T > C), age range: 16–42 years). DNA was isolated from peripheral blood mononuclear cells (PBMC). Genotyping of miRNA-27a rs895819 was detected using a TaqMan® SNP Genotyping assay.ResultsWe did not find a significant association of miR-27a polymorphism with PE susceptibility in our data. However, in the subgroup analysis (based in HIV status), the variant genotypes (TC/CC) were associated with higher body mass index (BMI) among PE women (32.57 vs. 29.25, p = 0.064), significantly in the presence of HIV infection (33.47 vs. 27.8, p = 0.005).ConclusionThe results of this study suggests that miR-27a rs895819 may not be associated with PE susceptibility; however, the miR-27a TC/CC genotype increases susceptibility to elevated BMI in PE, which may be significantly influenced by co-morbid HIV infection among pregnant women on HAART.

Highlights

Preeclampsia (PE) and HIV/AIDS present a major health challenge globally
After informed consent was obtained, participants were recruited over a 14-month period from July 2013 to September 2014 from the maternity unit at Prince Mshiyeni Memorial Hospital in Durban, South Africa
Of relevance is the observation that the variant TC/CC genotype is associated with higher body mass index (BMI) in the PE women studied, in contrast to lower BMI values in normotensive pregnancies

Summary

Introduction

Preeclampsia (PE) and HIV/AIDS present a major health challenge globally. South Africa has the highest disease burden of both HIV/AIDS and PE in the world. The pathogenic mechanisms underlying PE remain to be elucidated; immune maladaptation, inadequate placental development and trophoblast invasion, placental ischaemia, oxidative stress and thrombosis are all thought to represent key factors in the development of disease [7] All of these components have genetic factors that may be involved in the pathogenesis of PE [7]. MiRNAs regulate pathways in adipose tissue that control adipogenesis, insulin resistance and inflammation [8], and regulate endothelial cell function and angiogenesis by regulating pro- and anti-angiogenic activity [9] They have been shown to regulate vascular integrity in angiogenesis induced by ischemia [10, 11]

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