MicroRNA-275 and its target Vitellogenin-2 are crucial in ovary development and blood digestion of Haemaphysalis longicornis

Jiawei Hao,Hui Wang,Xiaocui Liu,Zhen Wang,Jianxun Luo,Feng Wu,Hong Yin,Jin Luo,Jinxia Guo,Ze Chen,Qiuyu Chen,Qiaoyun Ren,Guangyuan Liu

doi:10.1186/s13071-017-2153-1

Abstract

BackgroundThe hard tick Haemaphysalis longicornis is widely distributed in eastern Asia, New Zealand and Australia and is considered the major vector of Theileria and Babesia, harmful parasites to humans and animals. Female ticks need successful blood meals to complete the life-cycle. Therefore, elucidation of the underlying molecular mechanisms of H. longicornis development and reproduction is considered important for developing control strategies against the tick and tick-borne pathogens.MethodsLuciferase assays were used to identify the targets of micro RNA miR-275 in vitro. RNAi of Vitellogenin (Vg) was used in phenotype rescue experiments of ticks with miR-275 inhibition, and these analyses were used to identify the authentic target of miR-275 in vivo. The expression of miR-275 in different tissues and developmental stages of ticks was assessed by real-time PCR. To elucidate the functions of miR-275 in female ticks, we injected a miR-275 antagomir into female ticks and observed the phenotypic changes. Statistical analyses were performed with GraphPad5 using Student’s t-test.ResultsIn this study, we identified Vg-2 as an authentic target of miR-275 both in vitro and in vivo by luciferase assays and phenotype rescue experiments. miR-275 plays the regulatory role in a tissue-specific manner and differentially in developmental stages. Silencing of miR-275 resulted in blood digestion problems, substantially impaired ovary development and significantly reduced egg mass (P < 0.0001). Furthermore, RNAi silencing of Vg-2 not only impacted the blood meal uptake (P < 0.05) but also the egg mass (P < 0.05). Significant rescue was observed in miR-275 knockout ticks when RNAi was applied to Vg-2.ConclusionTo our knowledge, this study is the first demonstration that miR-275 targets Vg-2 in H. longicornis and regulates the functions of blood digestion and ovary development. These findings improve the molecular understanding of tick development and reproduction.

Highlights

The hard tick Haemaphysalis longicornis is widely distributed in eastern Asia, New Zealand and Australia and is considered the major vector of Theileria and Babesia, harmful parasites to humans and animals
Computational analysis showed that there were many potential binding sites in vitro The putative target genes (Vgs), but only two predicted results within the 7mer seed sequence sites in Vg-2 (Table 2)
The results suggested that miR-275 is predominantly involved in ovary development and blood digestion, further demonstrating that miR-275 has an important role in egg development

Summary

Introduction

The hard tick Haemaphysalis longicornis is widely distributed in eastern Asia, New Zealand and Australia and is considered the major vector of Theileria and Babesia, harmful parasites to humans and animals. Ticks are hematophagous ectoparasites that require blood meals from vertebrates to obtain a variety of nutrients. They transmit many vector-borne pathogens to humans and animals [1] and cause significant economic losses [2, 3]. Most miRNAs act on 3' untranslated regions (3' UTR) of the target mRNA [10], but they can bind to the 5' UTR [11, 12] and the open reading frame (ORF) [13, 14]

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Results

Discussion

Conclusion