Abstract
Renal cell carcinoma (RCC) is the most common type of kidney cancer in adults and is associated with a poor prognosis due to a lack of early‑warning signs, protean clinical manifestations, and resistance to radiotherapy and chemotherapy. Recently, increasing evidence has suggested that microRNAs (miRNAs) are involved in the proliferation, invasion and apoptosis of various types of human cancer cells. In a previous study, miRNA expression profiles from renal cell carcinoma (RCC) revealed that expression of miR‑20b‑5p was significantly downregulated in RCC tissues. The aim of this study was to investigate the expression and functional significance of miR‑20b‑5p in RCC. The expression of miR‑20b‑5p was quantified in 48 paired RCC tissues and cell lines, and compared with adjacent normal tissues and the 293T cell line by reverse transcription‑quantitative polymerase chain reaction. The functional impact of miR‑20b‑5p on cell proliferation, cell migration and apoptosis in the 786‑O and ACHN RCC cell lines, was determined by an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, a scratch assay and flow cytometry. To the best of our knowledge, the present study was the first to reveal that miR‑20b‑5p was downregulated in RCC tissues and cell lines. It also demonstrated that upregulation of miR‑20b‑5p inhibited cellular migration and proliferation, and promoted cellular apoptosis, suggesting that miR‑20b‑5p functioned as a potential tumor suppressor. However, further studies are required to fully determine the effects of miR‑20b‑5p and the miR‑20b‑5p‑mediated molecular pathway in RCC and other types of cancer. In conclusion, these results imply that miR‑20b‑5p may be a biomarker for early detection and prognosis prediction, as well as a therapeutic target for RCC.
Highlights
Renal cell carcinoma (RCC) is the most common type of kidney cancer in adults, accounting for ~90% of all renal tumors and 3.9% of all cancers [1,2]
To confirm downregulation of miR‐20b‐5p, Reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR) was performed in 48 paired RCC tissues and adjacent normal tissues
An aberrant miRNA expression signature is a hallmark of cancer, including kidney cancer [23]. miRNA profiling in kidney cancers revealed that a number of miRNAs were up‐ or downregulated, including miR‐20b, and specific miRNA profiles could serve as a valuable tool for diagnosis [19,25]
Summary
Renal cell carcinoma (RCC) is the most common type of kidney cancer in adults, accounting for ~90% of all renal tumors and 3.9% of all cancers [1,2]. In 2014, 63,920 novel cases and 13,860‐related fatalities from renal cancer are estimated to have occurred [3]. 25% of patients present with advanced disease when initially diagnosed with RCC, and 33.3% of the patients who undergo resection of localized disease have a recurrence [7]. Survival of patients with localized tumors who undergo radical nephrectomy is significantly longer than patients with regional and distant metastasis, underscoring the importance of early detection [8]. It is critical to identify novel molecular biomarkers for early detection, diagnosis and targeted therapy
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
