MicroRNA-200c suppressed cervical cancer cell metastasis and growth via targeting MAP4K4.

Abstract

To dissect the functioning mode of miR-200c on cervical cancer cell metastasis and growth and provide therapeutic targets for cervical cancer. By quantitative Real-time polymerase chain reaction, the miR-200c expression level in 42 pairs of cervical cancer tissue samples and six cervical cancer-derived cell lines were examined. Using miR-200c mimics, we analyzed the effects of miR-200c over-expression on cell proliferation, invasion, and migration. Dual-luciferase activity assay was recruited to examine the potential target gene MAP4K4 that predicted by several databases. Protein level was studied using Western blot. miR-200c expressed significantly lower in cervical cancer tissue samples and cell lines. And over-expression of miR-200c in cervical cancer cells significantly decreased the cell invasion, migration and proliferation abilities. Dual-luciferase and Western blot confirmed MAP4K4 as a target gene of miR-200c. Furthermore, up-regulation of MAP4K4 counteracted the suppressive effect of miR-200c over-expression on cell growth and metastasis. miR-200c could suppress cervical cancer cell proliferation and progression via regulating MAP4K4, which might provide a new target for cervical cancer diagnosis and therapy.