Abstract
BackgroundPolycystic ovary syndrome (PCOS) is one of the most common endocrine metabolic disorders characterized by hyperandrogenism, polycystic ovaries and ovulatory dysfunction. Several studies have reported that the aberrant expression of miRNAs contributes a lot to disordered folliculogenesis in PCOS, though the role and underlying mechanism of microRNA-200b (miR-200b) and microRNA-200c (miR-200c) in the development of PCOS remain unclear.MethodsThe expression of miR-200b in granulosa cells (GCs) derived from 90 PCOS patients and 70 controls was analyzed by using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Granulosa-like tumor cell line (KGN) was cultured for cell counting kit-8 (CCK-8) assays after over-expression of miR-200b, miR-200c or knockdown phosphatase and tensin homolog (PTEN). TargetScan was used to identify the potential targets of miR-200b and miR-200c, which was further verified by qRT-PCR, western blot and luciferase assays.ResultsSignificantly increased expression of miR-200b was observed in PCOS patients compared with the controls. Moreover, over-expression of miR-200b and miR-200c inhibited the proliferation of KGN cells. In addition, our results verified that miR-200b and miR-200c directly targeted PTEN, knockdown of which suppressed KGN cells proliferation.ConclusionOur findings demonstrate that miR-200b and miR-200c suppress the proliferation of KGN cells by targeting PTEN, and this might provide new evidence for abnormal proliferation of GCs in PCOS.
Highlights
Polycystic ovary syndrome (PCOS) is one of the most common endocrine metabolic disorders characterized by hyperandrogenism, polycystic ovaries and ovulatory dysfunction
Body mass index (BMI), Fasting plasma glucose (FPG), Fasting insulin (FINS), Luteotropic hormone (LH), T, Anti-Mullerian hormone (AMH) and Antral follicle count (AFC) were significantly increased in PCOS patients, while Follicle stimulating hormone (FSH) was dramatically decreased (P < 0.05, for all)
To determine whether miR-200b was associated with PCOS, Quantitative reverse transcription-polymerase chain reaction (qRTPCR) was used to assess the expression of miR-200b in granulosa cells (GCs) derived from 90 PCOS and 70 control women
Summary
Polycystic ovary syndrome (PCOS) is one of the most common endocrine metabolic disorders characterized by hyperandrogenism, polycystic ovaries and ovulatory dysfunction. Polycystic ovary syndrome (PCOS) is one of the most common endocrine metabolic disorders affecting about 5% women of reproductive age [1]. It is characterized by hyperandrogenism, polycystic ovaries and ovulatory dysfunction, and associated with insulin resistance,. Throughout oocyte development, it has been demonstrated that there is an interdependence between the oocyte and its surrounding GCs, the support of which is essential to provide the oocyte with He et al Reproductive Biology and Endocrinology (2019) 17:68 a suitable microenvironment, such as nutrients and growth regulators [10,11,12]. The purpose of this study is to explore whether miR-200b and miR-200c are involved in the abnormal proliferation of PCOS GCs and its underlying mechanism
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