MicroRNA-200 Family Promotes Neurite Outgrowth by Suppression of PTEN Expression in PC12 Cells and SCG Neurons

Abstract

Event Abstract Back to Event MicroRNA-200 Family Promotes Neurite Outgrowth by Suppression of PTEN Expression in PC12 Cells and SCG Neurons Qi Wu1 and Jun Wan1, 2* 1 Shenzhen-PKU-HKUST Medical Center, Biomedical Research Institute, China 2 The Hong Kong University of Science and Technology, Division of Life Science, China Neuronal differentiation is a key event during the brain development. The outgrowth of neurite from cell body of nerve cells is the first and critical step during neuronal differentiation. This process is tightly regulated by a plethora of signaling proteins. Recently, MicroRNAs have emerged as key posttranscriptional regulators of gene expression during neuronal development. Herein, we reported that a brain enriched microRNA family, micoRNA-200 family, including miR-200a/b/c, miR-141 and miR-429, are implicated in controlling neurite outgrowth in PC12 and rat superior cervical ganglia (SCG) neurons. Luciferase reporter assay and western blot analysis showed that each member of miR-200 family directly targets to 3'-UTR of Phosphatase and tensin homolog (PTEN) individually to inhibit its protein expression. Among them, the miR-200c is the major suppressor of PTEN expression. Either overexpression of miR-200c or knockdown of PTEN promotes NGF-induced neurite outgrowth in PC12 cells. In contrast, NGF-induced neuritogenesis of PC12 is blocked by knockdown of miR-200c or overexpression of PTEN, respectively. More interestingly, we also observed similar effects of miR-200c or PTEN during SCG neuronal differentiation in vitro. Taken together, our results demonstrated that miR-200 family plays an important role in neurite outgrowth by suppressing PTEN expression in PC12 cells or SCG neurons. Keywords: PC12 Cells, Pten, Neurite outgrowth, microRNA-200 family, SCG neurons Conference: XII International Conference on Cognitive Neuroscience (ICON-XII), Brisbane, Queensland, Australia, 27 Jul - 31 Jul, 2014. Presentation Type: Poster Topic: Cognition and Executive Processes Citation: Wu Q and Wan J (2015). MicroRNA-200 Family Promotes Neurite Outgrowth by Suppression of PTEN Expression in PC12 Cells and SCG Neurons. Conference Abstract: XII International Conference on Cognitive Neuroscience (ICON-XII). doi: 10.3389/conf.fnhum.2015.217.00374 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 19 Feb 2015; Published Online: 24 Apr 2015. * Correspondence: Dr. Jun Wan, Shenzhen-PKU-HKUST Medical Center, Biomedical Research Institute, Shenzhen, China, wanj@ust.hk Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Qi Wu Jun Wan Google Qi Wu Jun Wan Google Scholar Qi Wu Jun Wan PubMed Qi Wu Jun Wan Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.