MicroRNA-194-5p on Proliferation and Invasion of Laryngeal Cancer Cells by Regulating the Expression of SMURF1 and Activating the mTOR Signalling Pathway

Abstract

Laryngeal cancer has become the focus of research because of its high incidence rate and mortality rate. However, the research on microRNA-194-5p in laryngeal cancer is quite rare. The purpose of this study is to explore the effect of microRNA-194-5p on the proliferation and invasion of laryngeal cancer cells, in order to find an effective way to treat laryngeal cancer. The results showed that microRNA-194-5p could activate mammalian target of rapamycin signaling pathway by regulating the expression of smad ubiquitin regulatory factor 1, which had an effect on laryngeal cancer cells. The results showed that the absorbance of microRNA-194-5p group was 0.38 lower than that of negative control group, which indicated that up regulation of microRNA-194-5p could weaken the proliferation of laryngeal cancer cells. In addition, the average number of laryngeal cancer cells in negative control group and microRNA-194-5p group was 125.2 and 53.8 respectively, which indicated that microRNA-194-5p could reduce the number of laryngeal cancer cells passing through basement membrane and their invasion ability.