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Abstract
Purpose: microRNA-193a-5p is one of the well-known tumor suppressor miRNAs in the body but in many cases, its expression became reduced in patients suffering from gastric cancer (GC). The main purpose of this study was to restore the function of this miRNA in human GC cells and investigating the effects of enhanced expression of miR-193a-5p on proliferation, apoptosis, and migration of GC cells upon in vitro transfection. Methods: The KATO III GC cells were treated with 100 nM of miR-193a-5p or negative control sequences. Following that, the MTT assay, flow cytometry assay, and wound-healing assay were applied to estimate the impacts of enhanced expression of this miRNA on the viability, apoptosis, and migration rate of the cells, respectively. Moreover, the total RNA was isolated and alterations in the mRNA expression ratio of migratory genes were measured by qRT-PCR techniques. Results: The findings designated that enhanced expression of miR-193a-5p suppressed the migratory ability of the cells, but had no significant effects on cell survival or apoptosis of the transfected cells. In addition, this inhibitory function of miR-193a-5p on the migration rate of the KATO III cell line occurs with concurrent suppression of vimentin and MMP-9 gene expression. Conclusion: It can be concluded that miR-193a-5p negatively influences the migratory ability of the cancerous cells and restoring its effects can be regarded as a promising target of future therapeutic interventions, especially for GC metastasis.
Highlights
Gastric cancer (GC) is one of the most frequent types of cancers in the world that leads to t high rates of cancer-related mortality each year.[1]
It can be concluded that miR‐193a‐5p negatively influences the migratory ability of the cancerous cells and restoring its effects can be regarded as a promising target of future therapeutic interventions, especially for GC metastasis
2|Page expression of tumor suppressor miRNAs is greatly concerned in GC, in the current study, we tried to evaluate the effects of miR‐193a‐5p mimics on proliferation, apoptosis, and migration of the cells and investigate the expression of vimentin and MMP-9 genes in KATO III cell lines. miR‐193a‐5p may be a new target for the design of targeted therapy and may provide a potential biomarker to early detection and GC therapy
Summary
The findings designated that enhanced expression of miR‐193a‐5p suppressed the c migratory ability of the cells, but had no significant effects on cell survival or apoptosis of the ctransfected cells.
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