Abstract
microRNAs (miRNAs) are a class of short noncoding RNA molecules that have a critical role in the initiation and progression of types of human cancer, including prostate cancer. In the present study, the expression of miR-181 in prostate cancer tissues was evaluated and was demonstrated to be significantly upregulated in prostate cancer tissues compared with that in adjacent normal tissues. The results of in vitro MTT and BrdU incorporation assays, as well as cell-cycle analysis, indicated that miR-181 overexpression markedly promoted the proliferation of LNCaP cells. Furthermore, miR-181 overexpression was found to promote the progression of LNCaP tumor growth in nude mice. Mechanistic studies demonstrated that dosage-sensitive sex reversal, adrenal hypoplasia critical region, on chromosome X, gene 1 (DAX-1), a negative regulator of androgen receptor in prostate cancer, was inhibited by miR-181 overexpression. Therefore, the results from the present study suggest that miR-181 functions as a growth-suppressive miRNA during prostate cancer development.
Highlights
Introduction genes by targetingmRNAs, resulting in mRNA degradation or translation repression [5]
The results demonstrated that cell growth was significantly increased in miR‐181‐overexpressing cells compared with that of their corresponding controls, measured using the MTT and BrdU assays (Fig. 2B and C)
It has been previously demonstrated that several miRNAs are dysregulated in prostate cancer tissues or cell lines, and they have been shown to be associated with prostate cancer progression and disease outcome [11,12,13]
Summary
Introduction genes by targetingmRNAs, resulting in mRNA degradation or translation repression [5]. MiR‐888 is a miRNA secreted by prostate cells that promotes prostate cell growth and migration through the repression of the levels of protein produced by the tumor suppressor genes RBL1 and SMAD4 [6]. Previous studies have demonstrated that the upregulation of hepatic miR‐181 promotes the growth, clonogenic survival, migration and invasion of hepatocellular carcinoma cells [7,8]. The expression level of miR‐181 is significantly associated with overall survival in hematological malignancies and may be an important clinical prognostic factor for patients with hepatocellular carcinoma [9]. In the present study, the expression of miR‐181 was determined in prostate cancer tissues. The targets of miR‐181 were investigated in order to determine the underlying mechanism of miR‐181 in prostate cancer
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
