MicroRNA-155 promotes pro-inflammatory functions and augments apoptosis of monocytes/macrophages during Vibrio anguillarum infection in ayu, Plecoglossus altivelis

Abstract

Upon recognition of pathogen-associated molecular patterns by pattern-recognition receptors, immune cells are recruited, and multiple antibacterial/viral signaling pathways are activated, leading to the production of immune-related cytokines, chemokines, and interferons along with further activation of the adaptive immune response. MicroRNAs (miRs) play essential roles in regulating such immune signaling pathways, as well as the biological activities of immune cells; however, knowledge regarding the roles of miRs in the immune-related function of monocytes/macrophages (MO/MΦ) remains limited in teleosts. In the present study, we addressed the effects of miR-155 on Vibrio anguillarum-infected MO/MΦ. Our results showed that miR-155 augmented MO/MΦ expression of proinflammatory cytokines and attenuated the expression of anti-inflammatory cytokines. Additionally, the phagocytosis and bacteria-killing abilities of these cells were boosted by miR-155 administration, which also promoted M1-type polarization but inhibited M2-type polarization. Furthermore, the V. anguillarum-infection-induced apoptosis was also enhanced by miR-155 mimic transfection, which might have been due to excessive inflammation or the accumulation of reactive oxygen species. These results represent the first report providing a detailed account of the regulatory roles of miR-155 on MO/MΦ functions in teleosts and offer insight into the evolutionary history of miR-155-mediated regulation of host immune responses.