Abstract
Infection with Brucella is characterized by the inhibition of host immune responses. MicroRNA-155 (miR-155) has been implicated in the immune response to many diseases. In this study, its expression during Brucella 16M infection of macrophages and mice was analyzed. Expression of miR-155 was significantly induced in macrophages at 24 h post infection. Further, an analysis of infected mice showed that miR-155 was inhibited at 7 and 14 days but induced at 28 days. Interestingly, this trend in induction or inhibition was reversed at 7 and 14 days in 16M△virB-infected mice. This suggested that decreased expression of miR-155 at an early stage of infection was dependent on intracellular replication. In humans with brucellosis, serum levels of miR-155 were significantly decreased compared to those in individuals without brucellosis and healthy volunteers. Significant correlations were observed between serum level of miR-155 and serum anti-Brucella antibody titers and the sweating symptom. This effect suggests that Brucella interferes with miR-155-regulated immune responses via a unique mechanism. Taken together, data from this study indicate that Brucella infection affects miR-155 expression and that human brucellosis patients show decreased serum levels of miR-155.
Highlights
Infection with Brucella is characterized by the inhibition of host immune responses
A recent study showed that the infection of macrophage RAW264.7 cells with Brucella significantly altered miRNA expression profiles, suggesting that miRNAs are involved in interactions between Brucella and its hosts[11]
Expression levels of miR‐155 in macrophages are altered by Brucella infection
Summary
Infection with Brucella is characterized by the inhibition of host immune responses. MicroRNA-155 (miR-155) has been implicated in the immune response to many diseases. Expression of miR-155 was significantly induced in macrophages at 24 h post infection. Significant correlations were observed between serum level of miR-155 and serum anti-Brucella antibody titers and the sweating symptom This effect suggests that Brucella interferes with miR-155-regulated immune responses via a unique mechanism. Data from this study indicate that Brucella infection affects miR-155 expression and that human brucellosis patients show decreased serum levels of miR-155. A recent study showed that the infection of macrophage RAW264.7 cells with Brucella significantly altered miRNA expression profiles, suggesting that miRNAs are involved in interactions between Brucella and its hosts[11]. To probe the possible roles of miR-155 in Brucella infection, in the present study, we examined miR-155 expression during Brucella infection of macrophages and mice and analyzed serum levels of miR-155 in patients with brucellosis
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