Abstract
BackgroundThis study aims to investigate the effect of miR-10b overexpression on cancer cell proliferation, migration, invasion, and Hoxd10 expression.MethodsThe effect of miR-10b on proliferation, migration, and invasion of MKN-28, BGC-823, and SGC-7901 cells and the expression of Hoxd10 protein in SGC-7901 and BGC-823 cells were detected following transfection of miR-10b inhibitor or Negative Control B. Expression of Hoxd10 protein in 436 paraffin-embedded cancer tissues was also investigated.ResultsmiR-10b was significantly upregulated in AGS, MKN-28, BGC-823, HCG-27, SGC-7901, and MKN-45 cell lines, miR-10b inhibitor significantly inhibited proliferation and migration of MKN-45, BGC-823 and SGC-7901 cells 48 h after transfection, while Hoxd10 protein in these cells lines had increased 72 h after transfection. Hoxd10 was highly expressed in gastric cancer and correlated with size of tumor, Lauren classification, depth of invasion, lymph node and distant metastasis, Tumor-Node-Metastasis (TNM) stage, and prognosis.ConclusionsmiR-10b promotes migration and invasion through Hoxd10 in human gastric cancer cell lines and may play an important role in tumorigenesis, progression, and prognosis.
Highlights
This study aims to investigate the effect of miR-10b overexpression on cancer cell proliferation, migration, invasion, and Hoxd10 expression
Results miR-10b is upregulated in gastric cancer cell lines miR-10b was significantly upregulated in AGS, MKN-28, BGC-823, HCG-27, SGC-7901, and MKN-45 cell lines (4.7 × 10−4, 1.4 × 10−3, 1.3 × 10−3, 9.8 × 10−4, 8.3 × 10−4, and 3.7 × 10−4, respectively) compared with the nonmalignant gastric epithelial cell line GES-1 (3.2 × 10−5) (P < 0.05)
MiR-10b inhibited cell proliferation, migration, and invasion in vitro To study the biological role of miR-10b in gastric cancer, miR-10b inhibitor or Negative Control B was transfected into MKN-28, BGC-823, and SGC-7901 cells, and miR10b levels were evaluated by RT-PCR. miR-10b levels in SGC-7901, BGC-823, and MKN-28 cells transfected with miR-10b inhibitor were much lower than in Negative Control B-infected cells (P < 0.05), 24 h after transfection
Summary
This study aims to investigate the effect of miR-10b overexpression on cancer cell proliferation, migration, invasion, and Hoxd expression. MiRNAs play a role in cancer or cancer prevention by adjusting the expression of downstream mRNA [2]. Nothing is known about the role of miR-10b gastric cancer metastasis. In this study, we analyzed the level of miR-10b in human gastric cancer cell lines AGS, MKN-28, BGC-. 823, HCG-27, SGC-7901, 9811P, and MKN-45 and in the non-malignant gastric epithelial cell line GES-1, and the effect of miR-10b expression on gastric cancer cell proliferation, invasion, and migration. We aimed to elucidate the role of miR-10b in gastric cancer formation, development, invasion and metastasis, and its mechanism
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