Micropuncture investigation of the anion content of colloid from single rat thyroid follicles. A micromethod for the simultaneous determination of iodide and chloride in nanomole quantities.

Abstract

Micropuncture of rat thyroid follicles has been used to study the chloride and protein composition of the colloid. The mean Cl concentration in colloid was 97 mEq/l±20 (S.D.,n=30) and in plasma it was 110±6 mEq/l (n=6). The mean protein concentration in colloid was 25±16 g-% (n=37) relative to a porcine thyroglobulin standard, and in plasma it was 8.3±3.3 g-% relative to a bovine serum albumin standard.