Micropropagtion of two Strawberries (Fragaria anariassa L.) Cultivars

Abstract

Shoot tips from two strawberry (Fragaria anariassa L.) cultivars, Gabi and Edpeerenwere cultured on MS medium supplemented with 0, 0.5, 1.0, 1.5 or 2.0mg /1 of BA tostudy their effects on number, length, fresh weight and dry weight of shoots. Thestudy also included measuring the effect of different concentrations of IBA (0 , 0.1 ,0.3 , 0.5 or 1.0) mg / l on rooting percentage, roots number and length. This studyalso included the acclimatization processes for plantlets in sand and a mixture ofsand and peat moss media. Results indicated that addition of BA to the nutrientmedium led to a significant increase in all investigated traits in comparison with the control with obvious superiority of 2 mg /l which reflected positively on the meannumber of shoots (20.0) shoots / plant, the fresh and dry weight were 1237.73 and136.4 mg respectively, especially with Gabi cultivar, the result showed that all thelevels of IBA in the nutrient medium led to 100% rooting after 6 weeks of plantingof both cultivars compared with 50% of in control. It was also noticed significantincreases in the roots number and shoot lengths when IBA concentration wasincreased in the medium. The best growth for the rooted plantlets was found to be1:1 sand: peat moss which showed survival percentages of 80 and 90% after 4weeksof planting Erdpeern and Gabi cultivars respectively.