Abstract
Origanum acutidens (HAND.-MAZZ.) IETSWAART is a promising ornamental plant that can be widely used in landscape management. It is endemic to Eastern Anatolian region of Turkey. Tissue culture has not been used to micropropagate it. The study reports stem node explants from one-week-old seedlings of the plant for successful micropropagation. The stem nodes were cultured on MS medium containing 0.6, 1.2, 1.8, and 2.4 mg/L BAP with 0.2 mg/L NAA. Visible effects of culture media on shoot proliferation were recorded. Shoot regeneration rate was maximum on MS medium containing 1.80 mg/L BAP-0.2 mg/L NAA. The micropropagated shoots were rooted on MS medium containing 0.2 mg/L NAA. All microrooted plantlets survived during acclimatisation on peat moss. It was concluded that O. acutidens can be successfully micropropagated under in vitro conditions.
Highlights
Origanum acutidens (HAND.-MAZZ.) IETSWAART is a perennial herbaceous plant endemic to Eastern Anatolian region of Turkey and has beautiful small light pink to white flowers that open during July-August period each year [1]
The results showed that different concentrations of BAP-0.2 NAA mg/L are effective combinations for the regeneration of shoots from stem node explants
Maximum shoot regeneration percentage was recorded on MS medium containing 1.8 mg/L BAP-0.2 mg/L NAA and 2.4 mg/L BAP-0.2 mg/L NAA
Summary
Origanum acutidens (HAND.-MAZZ.) IETSWAART is a perennial herbaceous plant endemic to Eastern Anatolian region of Turkey and has beautiful small light pink to white flowers that open during July-August period each year [1] They grow on limestone and calcareous rocks and slopes need very little water for their growth and maintenance. Overexploitation of the plant from natural resources is acting negatively on the populations of the plant and large reserves of the plant that were visible a few years back are no longer visible these days [3] This suggests that the plant could be used as ornamental in landscaping, pharmaceutical, or food industry after development of protocols for its multiplication through traditional or modern biotechnological methods. The study aimed to develop an efficient mass proliferation protocol O. acutidens using stem node explants, which has never been reported earlier
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
