Micropropagation of White Palash tree (Butea monosperma (Lam.) Taub. Var. lutea (Witt.)).

D Rathnaprabha ,Raghu E,N Muralikrishna ,Yashodhara ,Anguraj Sadanandam

doi:10.19071/jp.2016.v8.3070

Abstract

An efficient and reproducible protocol is established for rapid in vitro multiplication of an endangered, valuable medicinal plant, Butea monosperma (Lam.) Taub. Var. lutea, through cotyledonary nodes of mature seeds. Among various cytokinins tested, high frequency of direct shoot regeneration was induced on Murashige and skoog (MS) medium supplemented with BAP, which found to be more effective and showed optimal response at 2 mg/L with a maximum number of 8.35±0.32 multiple shoots per explant. Proliferation of shoots was established by repeated subculturing on to same regeneration medium with 2-3 weeks of time interval. Rooting of regenerated shoots was achieved after 3 weeks of culture on MS medium containing 1 mg/L IBA. In vitro raised plantlets were transferred to pots containing sterilized soil and vermiculate mixture in 1:1 ratio and then shifted to greenhouse. Well established plantlets exhibited 75% survival rate.

Highlights

Butea monosperma (Lam.)Taub.Var.lutea, belonging to the family Fabaceae, is an endangered, valuable, woody plant
After removal of the radicle and primary shoots, the cotyledonary nodes were inoculated in 300 mL screw capped glass jars containing Murashige and Skoog (MS) medium supplemented with 0.5-3.0 mg/L of N6-benzylaminopurine (BAP), kinetin (KN), and thidiazuron (TDZ)
The application of micropropagation techniques to a range of plant species has been increased with interest to excised cotyledonary node as suitable experimental material, including Acacia nelotica (Dewan et al, 1992), Dalbergia sissoo (Pradhan et al, 1998), Sterculi aurens (Purohit and Dave, 1996), and Sesbania rostrata (Jha et al, 2004)

Summary

INTRODUCTION

Butea monosperma (Lam.)Taub.Var.lutea, belonging to the family Fabaceae, is an endangered, valuable, woody plant. The distribution of this species has been limited in area that needs immediate conservation This tree species is reported around Sabarkantha of Gujarat (Hitesh and Patel, 2015), Aurangabad in Maharashtra, and in few regions of Telangana such as Sircilla forests of Karimnagar, Peddagutta of Nizamabad, and Mallakpally of Warangal district (Reddy et al, 2008). Conventional propagation of this species through seeds has limited scope for large scale propagation because of its poor seed germination percentage, and enhanced in vitro seed germination protocol for its conservation has been developed with the highest percentage (65%) (Mahender et al, 2014). The authors described a simple, reproducible, and improved in vitro regeneration protocol of B. monosperma var. lutea through cotyledonary node explants derived from axenic seedlings

MATERIALS AND METHODS

RESULTS AND DISCUSSION

CONCLUSION