Abstract
The usage of the method of clonal micropropagation of plants is currently the most promising for obtaining virus-free plant material, including clonal rootstocks of stone fruit cultures. The introduction of clonal rootstocks of stone fruit cultures PK SK1, PK SK 2, AI 1 into invitro culture is best doneduring the active shoot growth period in the second decadeof May. During this period, the highest level of explants regeneration was noted: in the rootstock PK SK 1 – 94,6 %, in the rootstock AI 1 78.2%, in PK SK 2 80.4%, in Gisela 5 (control) 85,7%. From the third decade of May, the survival rate of explants begins to decline. At the stage of multiplication on the Murashige-Skoog medium, with the addition of 6-BAP 1 mg/L, the average multiplication factor of clonal rootstocks to the fourth passage was 1: 12 for PK SK1, for Gisela 5 and for AI 11: 8.
Highlights
The rootstock plays a significant role in the formation of crop yields, it determines the time when the trees enter into fruiting, the general state of plants, and the duration of the garden [1]
For successful clonal micropropagation of fruit crops on artificial nutrient media, the timing of the introduction of explants into in vitro culture is of great importance
In order to determine the optimal timing for the introduction of clonal rootstocks PK SK 1, PK SK 2, AI -1 and Gizela 5 into in vitro culture, explants were selected in several periods
Summary
The rootstock plays a significant role in the formation of crop yields, it determines the time when the trees enter into fruiting, the general state of plants, and the duration of the garden [1]. In the modern conditions of intensification of fruit production, the need for dwarf rootstocks is increasing, which will provide restrained plant growth, a compact crown of trees, thereby facilitating maintenance and harvesting [2]. Currenttechnology of growing fruit crops is impossible without the use of a healthy planting material. When growing stone fruit crops on clonal rootstocks, it is easier to obtain virus-free planting material [2]
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