Abstract
The Far East medicinal plant - <em>Plantago camtschatica</em> was propagated in vitro from tips of shoots (obtained in vitro) and from different explants of 4-week-old seedlings: seedling tips, hypocotyls, cotyledons, roots, first leaves. To our knowledge there is no information in literature about in vitro culture of this plantain. MS basal medium, supplemented with 0.6 pM IAA in combination with various cytokinins (BA, KIN, ZEA), was used. After 6 weeks of culture, micropropagation rate (MR) - mean number of buds and shoots per explant - was calculated. Our study proved that <em>P. camtschatica</em> species was amenable to propagation in vitro from different kinds of explants. However, multiplication by adventitious shoot regeneration from hypocotyl explants was found to be the most suitable method for the propagation of this plant. Adventitious shoots could root without stimulation what allows to omit the stage of rooting. The plants obtained as a result of micropropagation were not phenotypically changed.
Highlights
Kamchatic plantain Plantago camtschatica Link, syn
P. depressa Wild. subsp. camtschatica (Cham. ex Link) Pilg. is one of the East Asian species belonging to the Plantaginaceae family
Some other species of the genus Plantago were studied in culture in vitro: P. afra L. (Sarihan et al 2005), P. asiatica L
Summary
Kamchatic plantain Plantago camtschatica Link, syn. P. depressa Wild. subsp. camtschatica (Cham. ex Link) Pilg. is one of the East Asian species belonging to the Plantaginaceae family. auxin: 0.6 μM IAA, cytokinins: 2.2, 4.4 or 8.9 μM BA for shoots formated from tips, 8,9 μM BA, 9.3 μM KIN or 9.1 μM ZEA for shoot regeneration through organogenesis on hypocotyls, cotyledons, roots and first leaves. In our earlier study in the case of seedling tips for the other Far East taxon of genus Plantago P. asiatica MR was 5.18±0.58 for the same conditions of in vitro culture as in the present study for P. camtschatica (Makowczyñska and Andrzejewska-Golec 2003).
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