Abstract
Pistacia lentiscus L., belonging to Anacardiaceae family, is a typical species of the Mediterranean maquis and it is widely grown in Greece and Italy mainly for its aromatic resin extraction or as ornamental plant and also as Pistacia vera L. rootstock. Its propagation is difficult either by seed or by cuttings. The current study was carried out to optimize the micro propagation of Pistacia lentiscus L. starting from seedlings and woody explants. For the surface sterilization two different protocols were evaluated for woody explant and 6 treatments with combinations of different sterilizing agent types and concentrations were used for mature seeds. For woody explants, no significant differences could be evidenced on contamination percentage and plant survival but the initial growth in vitro of the explant was better in case of the first treatment (1.5% NaOCl for 30 min and 70% Ethanol for 1 min) than opposite combination. The highest seed contamination percentage occurred in case of treatment with 1% NaOCl for 30 min. The treatment with Ethanol (70%) for 30 second followed by three times washing with distilled water then use of NaOCl (1%) for 30 min permitted to obtain 100% of sterility. The highest seed germination (100% after 3 days) was obtained in seeds treated with Ethanol (70%) for 30 second then NaOCl (1%) for 30 min. In order to study the proliferation two different procedures were compared in liquid and agar-based media. Our results proved that proliferation rate increased 6.5 % by forced ventilation system. Longer shoots (10.5 cm) were obtained in temporary immersion system using RITA boxes. This culture system induced also the highest shoot weight which is the increasing of the 29.56% respect common vessels and agar-based medium
Highlights
The lentisk, known as Mastic tree, (Pistacia lentiscus L.), belonging to Anacardiaceae family, is grown widely in several Mediterranean countries like Greece and Italy (Zohary, 1952)
The effects of sterilization method on phenolic compound exudation of woody explants After 5-7 days from initial establishment, at the base of the Lentiscus woody explants, exudation of phenolic compounds was evidenced together with media in browning and this was the main inhibitor factor for explant establishment. in current experiment exudation of phenolic compounds were not observed as a consequence of different sterilization treatments
The explant initial growth expressed in elongation and initial development of the buds was affected by treatments at P≤ 0.05, the explants following the treatment 1 grew in the 20.87% respect to the 6.68% after the other one
Summary
The lentisk, known as Mastic tree, (Pistacia lentiscus L.), belonging to Anacardiaceae family, is grown widely in several Mediterranean countries like Greece and Italy (Zohary, 1952). Based on Sen et al (2013) reports, growth and development of explants were not negatively affected by NaOCl treatment, and other procedures of NaOCl treatment involve increased concentration and duration were less efficient in respect to sterilization, because of negative consequences on growth of plantlets. They reported that different concentration of sterilizing agents had different effects on contamination level and survival percentage. In current study in order to develop an efficient micro propagation method for the Lentisk plant, using RITA boxes, a growth comparison versus agarbased media were assessed
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