Abstract
The objective of this work was to evaluate asepsis protocols, composition and concentration of culture media in the in vitro establishment of three physalis species. In experiment I, seeds of 3 species were used x 3 asepsis protocols. In experiment II, 3 culture media and explants 3 species were used. In experiment III, explants of 2 species x 4 concentrations of MS culture medium were used. In experiment IV, explants of 2 species x 4 sucrose concentrations were used. For experiments II, III and IV after 30 days were evaluated some phytotechnical parameters. The experimental design was completely randomized, in a 3 x 3 (experiments I and II) and 2 x 4 (experiments III and IV) factorial scheme. Protocols II and III were appropriate for P. peruviana germination, and I and III for P. minima, the three protocols were efficient for controlling fungi and bacteria. The MS is the most suitable for the in vitro establishment of three species. The culture medium at 100% concentration obtained better values in the in vitro development parameters evaluated for the species P. peruviana and P. minima. Sucrose concentrations close to 50 and 20 g L-1 favored the establishment of P. peruviana and P. minima.
Highlights
Physalis is considered an exotic fruit with great economic potential, due to its adaptation to different Brazilian edaphoclimatic conditions
Considering the complexity of the micropropagation steps that involve several factors, adjustments to the in vitro multiplication protocols that already exist for the different Physalis species are necessary, so this study aimed to evaluate protocols of asepsis, composition and concentration of culture media in the establishment and multiplication of Physalis species in vitro
Experiment IV In figure 1 the results found when physalis species (P. peruviana e P. minima) were submitted to the concentration of sucrose are shown
Summary
Physalis is considered an exotic fruit with great economic potential, due to its adaptation to different Brazilian edaphoclimatic conditions. Its presence in supermarkets and local markets shows a trend of consumption by the population. Because it has high added value, fruit production is an alternative for small products that constitute the highest income per production area (Negri et al, 2016). Asexual propagation is the main form of physalis multiplication, since this fruit has a large amount of seeds and a high germination rate, it provides plantlets with high genetic variability, resulting in plants with different growth, vigor, performance and production of fruits, characteristics that are not interesting for commercial orchards (Oliveira et al, 2013). Among the challenges of this technique is mastering the various stages such as establishment and multiplication in vitro. (Mascarenhas et al, 2019)
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