Abstract
Phalaenopsis spp. was regularly produced through micropropagation by protocorm like bodies (PLBs); micropropagation takes a lot of labor, and has high cost of seedlings, energy and material. The purpose of this paper was to study the new technique of using in vitro embryogenesis culturing for microprogation. The method involved using protocorm like bodies as planting materials. PLBs were cut into slices and placed on the medium for callus initiation. The callus was initiated on the medium MS + BA (0.1 mg/l) supplemented with NAA (1 mg/l) or 2,4D (1 mg/l) and was proliferated on the medium MS + BA (0.1 mg/l) supplemented with NAA (1 mg/l). Somatic cell suspensions were initiated and proliferated on the medium MS + BA (0.1 mg/l) supplemented with NAA (0.5, 1 mg/l). Somatic cell suspensions were differentiated to embryonic cell suspensions on the MS medium supplemented with NAA (0.1 mg/l) + BA (0.5 mg/l). Embryonic cell suspensions were plated and regenerated on the medium: 1/2MS supplemented with NAA (0.1 mg/l) + BA (0.5 mg/l). Micropropagation of Phalaenopsis sp. via the embryogenesis technique was set up to produce 5,800 plantlets per one liter of somatic embryogenesis suspension.
Highlights
Traditional breeders that breed Phalaenopsis nowadays have a common problem that microbiological laboratories often face, that is that tissue culture often grows slowly, and it involves a lot of work to produce seedlings with at a high volume (Mamood, 1993)
The results showed that (Table 7): After 30 days of culture the somatic cell suspension was formed on a suspension culture supplemented with NAA (1 mg/l) or 2.4D (1 mg/l)
The callus produced on MS + BA (0.1 mg/l) supplemented with NAA (1 mg/l) or 2.4D (1 mg/l) and was cultured in medium on MS + BA (0.1 mg/l) + NAA (1 mg/l)
Summary
Traditional breeders that breed Phalaenopsis nowadays have a common problem that microbiological laboratories often face, that is that tissue culture often grows slowly, and it involves a lot of work to produce seedlings with at a high volume (Mamood, 1993). The somatic embryo propagation system solves the above problems with the advantages of: rapid multiplication in the form of cells, cloned embryos are a differentiation with a high regeneration coefficient, lower labor costs and price (AitkenChristie et al, 1994). The initial material in cloned culture has the role of ensuring parental traits and maintaining a high rate of regeneration over long periods of time in orchids (Lin et al, 2000). The appropriate somatic embryo culture medium plays an important role in the growth and differentiation of embryogenic cells (Arditii & Ersnt, 2004) and controls the differentiation and regeneration of embryos under the influence of the growth regulator (Ishii et al, 1998 & Huan et al, 2004). This article studies the rapid multiplication of Phalaenopsis by somatic embryo technology
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