Abstract
Pachysandra procumbens, the Allegheny pachysandra, is very rare to rare throughout most of its native range. Winter color, growth habit and ease of maintenance all recommend this perennial as an alternative ground cover for shady habitats. Development of micropropagation protocols may allow for its mass distribution. Non-wild collected shoots were disinfested using conventional procedures and were cultured and maintained in an MS based stock medium. Shoots proliferated equally well on an MS, a modified MS or a DKW based medium. Shoots had significantly more swelled buds when cultured in medium gelled with Gelrite or in liquid medium on membrane rafts compared to vermiculite. Microcuttings with or without a basal node rooted equally well. Microcuttings with or without an apical bud rooted equally well; however, microcuttings with an apical bud produced significantly longer roots.
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