Abstract
ABSTRACT Lavender (Lavandula dentata) has several medicinal applications, both in the fresh form and after industrial processing. Productivity increase could be obtained through the mass propagation, expanding the possibility of obtaining raw material for production of essential oil. The objective of this work was to develop a micropropagation protocol from explants obtained from seeds. Aspects of in vitro germination, multiplication, elongation and rooting were evaluated and, in sequence, the acclimatization process as well. The in vitro germination of lavender seeds can be obtained using a medium composed of agar and water. For multiplication, the use of MS medium (Murashige & Skoog, 1962) added with 5.0 µM 6-benzylaminopurine to the nutrient medium is the most appropriate. For elongation and rooting, the use of growth regulators is unnecessary. Acclimatization can be obtained directly by transferring the explant to plastic bags with substrate, kept in a greenhouse. It was possible to develop a protocol for the in vitro cultivation (establishment, multiplication, elongation, rooting and acclimatization) of lavender from explants of seed origin.
Highlights
A lavanda (Lavandula dentata) possui diversas aplicações medicinais, tanto in natura como após processamento industrial
The global market for vegetable essential oils grew by 7.5 billion dollars a year through 2018
The genus Lavandula has essential oils of medicinal properties related to the control of acne, cramps, flatulence, in addition to the relaxing and antibacterial actions
Summary
A lavanda (Lavandula dentata) possui diversas aplicações medicinais, tanto in natura como após processamento industrial. Foi possível desenvolver um protocolo para o cultivo in vitro (estabelecimento, multiplicação, alongamento, enraizamento e aclimatização) de lavanda a partir de explantes de sementes. The genus Lavandula has essential oils of medicinal properties related to the control of acne, cramps, flatulence, in addition to the relaxing and antibacterial actions. Some researches about the in vitro cultivation of Lavandula spp. have been carried out, to produce homogeneous plant material. With other species of the Lavandula genus, a protocol for L. angustifolia in vitro cultivation from young segments was possible from seeds’ germination. With the increase in the demand for new plant products, tissue culture can contribute to obtain bioactive compounds of interest (Dias et al, 2016) from the in vitro cultures
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