Micropropagation of interspecific hybrids of Vitis spp. in microenvironments with different gas exchanges

Abstract

The use of microporous membranes has provided improvements in the micropropagation of several plant species, resulting in greater survival when acclimatized. However, there is still little information on the use of microporous membranes in the micropropagation of vines, which needs improvement, due to the low survival of seedlings when acclimatized, being a barrier mainly for breeders who depend on this tool to propagate hybrids and Vitis species spp. In this sense, the objective of this work was to investigate the influence of two in vitro environments present and absent of microporous membranes on the micropropagation of three interspecific hybrids of Vitis spp. through morphophysiological and bivariate analyses. A completely randomized design arranged in a 3 × 2 factorial scheme (three hybrids CH.1.2, CH.3, and CH5.1; two ventilation systems with a polypropylene cover with and without the membrane with two layers of a microporous tape and one of polytetrafluoroethylene) was used. It was possible to verify if the factors (sealings x hybrids) in vitro interacted in the viability and affected the acclimatization phase of the acclimatized vines. For hybrid CH1.2 the interaction with the microenvironment present in membranes was not positive, attributing 60% survival to plants grown in lids without membranes. However, the use of microporous membranes reduced CO2 accumulation, providing an in vitro environment with a lower humidity rate. These environmental conditions favored the survival of CH5.1 CH1.3 hybrids, which was 100% when acclimatized. Therefore, the use of the membrane benefits micropropagation as well as the survival of vines when acclimatized.