Abstract

Gas plant is an elegant ornamental which blooms in early summer. It is commonly propagated by seed which is limited by a complex dormancy. Micropropagation was investigated as an alternative method for efficiently propagating gas plant. Rapidly growing two node stem pieces were taken from mature greenhouse grown plants, surface sterilized and placed in MS medium with 1 μM benzyladenine (BA). Shoot multiplication was studied using in vitro derived four node explants cultured on MS media containing BA at 0, 1, 5, or 10 μM. Explants cultured on BA at 1 and 5 μM averaged 3.9 and 5.6 shoots per explant respectively and a mean yield of 2.7 and 2.5 shoots greater than 2 cm in length. Explants cultured at 10 μM produced 5.7 shoots per explant with only 0.8 shoots greater than 2 cm in length. Microcuttings failed to root in vitro even in the presence of indolebutyric acid (IBA). Microcuttings directly stuck ex-vitro in a peat-lite medium root at 90% with 1.9 roots per cutting.

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