Abstract
Micropropagation (shoot tip and nodal cuttings culture) was used for the rapid propagation of the non-invasive, decorative, native plants of maiden pink (Dianthus deltoides L.) in order to preserve their genetic diversity. In vitro culture was successfully established on Murashige and Skoog medium (MS) using seeds as the initial material. In the shoot multiplication phase, the explants were cultured on MS medium supplemented with different concentrations of 6-benzylaminopurine (BAP) and naphthaleneacetic acid (NAA). The highest multiplication rate was achieved on a medium containing 0.1 mgL-1 of BAP and 0.1 mgL-1 of NAA. The rooting was successful on a hormone-free medium (100%), and the highest percentage of microplant acclimatization (97%) was recorded in a 4: 1 mixture of peat and sand.
Highlights
Plants within the genus Dianthus are popular with many gardeners
Because of its low maintenance requirements, and the fact that it is a native species that fits into a natural environment without adverse impacts on the surrounding natural ecosystems, D. deltoides is suitable for planting in Serbian mountain resorts
The characteristics of D. deltoides are in accordance with the increasingly dominating trend of naturalistic garden design that involves wild plant species (Rappaport, 1993)
Summary
Plants within the genus Dianthus are popular with many gardeners. D. deltoides is a spreading plant, growing to 30 cm in height. Single blooms appear in shades of pink and rose, cultivars are available in salmon, white and crimson. These plants make perfect borders or edgings where a semi-trailing plant is needed. D. deltoides is not invasive and it can be controlled when planted around the outer edges of landscapes. These plants can be used in rock gardens (Nau, 1996). For all these reasons, because of its low maintenance requirements, and the fact that it is a native species that fits into a natural environment without adverse impacts on the surrounding natural ecosystems, D. deltoides is suitable for planting in Serbian mountain resorts. The characteristics of D. deltoides are in accordance with the increasingly dominating trend of naturalistic garden design that involves wild plant species (Rappaport, 1993)
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