Abstract
We report in this work an in vitro propagation method for Dalbergia nigra using nodal segments from germinated seeds in vitro. Plant material was cultivated on MS medium supplemented with 6-benzyladeninepurine (BAP: 0 μM, 1.11 μM and 2.22 μM) and Naphthaleneacetic acd (NAA: 0.27 μM), combined with different amounts of subcultures for induction of shoot multiplication. Multiplied shoots were inoculated in MS and ½ MS medium (half strength of MS salts) to root, supplemented with NAA (0 μM and 4 μM). Data were analyzed by non-parametric statistical Kruskal-Wallis test. Best results to shoot multiplication were obtained using 1.11 μM or 2.22 μM of BAP combined 0.27 μM of NAA in multiplication medium and performing three subcultures, providing higher rates of shootsproduction per explant (average of 2.24) and high survival rate. Best results for rooting of shoots generated were obtained using a rooting medium containing ½ MS supplemented with 4 μM of NAA, providing a higher percentage of shoot rooting (average of 68%).
Highlights
IntroductionKnown as jacarandá-da-bahia, has originated in the Brazilian Atlantic Forest biome
Dalbergia nigra, known as jacarandá-da-bahia, has originated in the Brazilian Atlantic Forest biome
Best results for rooting of shoots generated were obtained using a rooting medium containing 1⁄2 MS supplemented with 4 μM of Naphthaleneacetic acid (NAA), providing a higher percentage of shoot rooting
Summary
Known as jacarandá-da-bahia, has originated in the Brazilian Atlantic Forest biome. This biome has already undergone several phases of exploration, with logging being one of the main responsible causes of severe damage, destroying large areas of forest (FEARNSIDE, 2006; GONZAGA, 2006). Micropropagation of Dalbergia nigra, an endangered Brazilian forest tree plant micropropagation have shown great utility in the production of seedlings of several economically important or endangered forest species (MARTIN et al, 2006, SANJAYA; RATHORE; RAI, 2006, TRIPATHI; KUMARI, 2010), where in vitro periodic subcultures of a single explant can generate numerous forest seedlings (BONGA; VON ADERKAS, 1992). The present work aimed to define a rapid and reproducible method for the in vitro propagation and multiplication of Dalbergia nigra for seedling production
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