Abstract
The effects were studied of light intensity, culture method and cytokinins on plant formation from callus-derived rhizomes of Cymbidium ensifolium var. misericors. The results demonstrated that one piece of rhizome produced seven shoot buds in 45 d when cultured in 1/2 MS basal liquid medium supplemented with 0.17 μM N6-(2-isopentenyl) adenine, 0.17 μM thidiazuron, 33 μM 6-aminopurine adenine and 1.5 μM naphthaleneacetic acid under 10 μmol m−2 s−1 artificial light and agitated at 60 rpm on a rotary shaker. These shoot-cm plantlets in 5 mo. when transferred to the same Gelrite-gel basal medium supplemented with 50 g l−1 banana pulp. Plantlets were acclimated and grew well when potted in the greenhouse.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
