Abstract
Adventitious budding from embryonic cotyledons of immature seeds of carob was obtained. The combination of BAP (4.44 μM) and NAA (1.5 μM) furthered the neoformation of adventitious buds. These latter were multiplied on MS medium added with BAP (2.22 μM). Stems and leaves growing were improved by adding 2.02 μM GA3. Elongation was favored by 0.5 μM NAA. 70% of rooting was obtained with 10 μM IBA.
Highlights
Open AccessCarob tree (Ceratonia siliqua L., Fabaceae) is an agro-sylvo-pastoral species with socio-economic and ecological interests
Adventitious budding from embryonic cotyledons of immature seeds of carob was obtained
Micropropagation techniques offer an alternative to the propagation of carob trees in order to satisfy the increased demand for this tree [6]
Summary
Carob tree (Ceratonia siliqua L., Fabaceae) is an agro-sylvo-pastoral species with socio-economic and ecological interests. The propagation of carob trees has been achieved by grafting young trees with selected female buds of productive trees [4]. The carob tree can be propagated by seedlings, but the seeds do not show correct germination rates unless after scarification; this multiplication does not guarantee the sex and the genetic characteristics of the cultivars. Cuttings multiplication is problematic because rooting is difficult [5]. In this context, micropropagation techniques offer an alternative to the propagation of carob trees in order to satisfy the increased demand for this tree [6]
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