Micropropagation of carnation (Dianthus caryophyllus L.) in liquid medium by temporary immersion bioreactor in comparison with solid culture.

Abstract

Developing scale-up system and automation of micropropagation in a bioreactor has been a possible way of cost reduction and intensive manual handling. We report a comparison between the results of experiments aimed at improving carnation micropropagation using new bioreactor according to Temporary Immersion Bioreactor (TIB) and solid culture. By applying different levels of BAP, at the concentration of 3 mg L−1, we observed 14.3 new shoots in TIB, but the number of new shoots on solid medium reached to 5.7 at the same treatment. Our results also showed that with 3 mg L−1 BAP in TIB, the initial fresh weight of plant material increased from 10 g to 450 g after 15 days. It is concluded that TIB showed more than 10 times shoot production of carnation. Shoot elongation and rooting induction was successfully stimulated in TIB by applying 1 mg L−1 IBA. Rooting of proliferated plantlets from TIB and solid culture were successfully happened, and led to highest number of roots (4.6 cm) and highest length of roots (6.87 cm) in TIB. More than 90% of plantlet was acclimatized to ex vitro. Our results suggested that mass production of carnation shoots in our simple TIB, with effective result, can be considered as a critical first step toward large scale production of carnation.