Abstract
Camptotheca acuminata (Chinese happy tree, Nyssaceae) is a source of the anticancer phytochemical, camptothecin. An endangered species in its native China, Camptotheca has been grown in this country on an experimental basis, but the germplasm base is extremely narrow. As a prelude to the establishment of a plant improvement effort designed to increase the efficiency of camptothecin production, in vitro studies that will enable plant regeneration and shoot proliferation from selected clones have been undertaken. Shoot proliferating cultures were established from shoot tip explants and were maintained on WPM medium containing 4 μm BA. MS medium and nodal explants proved unsatisfactory. Shoot proliferation was highest when in vitro shoot tips were cultured on 4 μm BA compared to media containing no growth regulator or the cytokinins zeatin, thidiazuron, or kinetin. In vitro—produced shoot tips were rooted by direct sticking in plastic containers filled with RediEarth mix, and were successfully adapted to the greenhouse environment.
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