Abstract
Factors affecting multiple shoot induction from single shoots and cotyledonary nodes of buckwheat seedlings, and from single shoots excised from multiple shoots were examined. More multiple shoots were induced from single shoots than from nodes derived from 1-week-old seedlings. Single shoots excised from multiple shoots further proliferated multiple shoots in half strength of modified Murashige & Skoog's medium containing 20μM benzylamino purine, 30 g/l sucrose, and 0.15g/l Gellan Gum at 6 week intervals. The multiplication rate was about 20. It was calculated that 208 shoots could be propagated from a single shoot per year. Plantlets were regenerated by rooting from single shoots in the medium containing 1μM indolebutyric acid and the rooting rate was about 60%.Acclimatization of in vitro plantlets was achieved by culturing in large vinyl bags for 10 days at about 65% acclimatization rate. Micropropagation procedure for buckwheat was established and the application and improvement of the technique are discussed.
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