Abstract
Polyphenols are a large diversity of chemical types and interactions that can be responsible for a multiplicity of protective functions ranging from toxicity and light/UV shielding to signal transduction. Bacharis antioquensis has been described as a potential source of new photoprotective compounds with antioxidant capacity associated to polyphenols compounds. The aim of the present work was to develop a micropropagation protocol of B. antioquensis and evaluate the production of polyphenols by in vitro plants exposure to UVB radiation. Branches in juvenile stage of B. antioquensis were collected, desinfected and cultured on half strength Murashige and Skoog medium, supplemented or not with growth regulators (TDZ, BA or GA3) on light/darkness conditions and liquid/solid media. After UV treatments, the absorption coefficient in the UVA-UVB range, the antioxidant capacity and the total phenol content (TPC) from all tissue cultures and the wild tissue were evaluated. Growth regulators, light conditions and type of culture medium (solid or liquid) had a favorable effect on the response of explants. Treatments containing BA + GA3 regulators (2 and 0.5 mg/L respectively) and TDZ (0.5 mg/L) showed positive results in bud growth in liquid medium and darkness. Results showed that UVR exhibited promoting effects on the accumulation of polyphenols, enhancing the absorption coefficient in the UVA-UVB range, the antiradical capacity and the TPC of B. antioquensis in vitro plants. Rev. Biol. Trop. 66(2): 754-764. Epub 2018 June 01.
Highlights
Plants synthesize a diversity of low molecular weight, structurally complex chemical compounds, commonly known as secondary metabolites (Moore, Andrew, Külheim, & Foley, 2014) These are present mainly under stress conditions, but their absence does not cause harmful effects to the plants (Edreva, 2005; Edreva et al, 2008; Khan, Aliabbas, Kumar, & Rajkumar, 2009)
Biotic and abiotic elicitors, which are classified on their origin, have been considered as an effective way to stimulate secondary metabolites due to the fact that both plant defense mechanism and metabolite production are interrelated via secondary metabolism (Manaf, Rabie, & Abd El-Aal, 2016)
Because phenolization reactions were observed in tissue plant, antioxidant compounds such as ascorbic acid (AA), citric acid (CA) and PVP were added to the culture in order to stop the reaction
Summary
Plants synthesize a diversity of low molecular weight, structurally complex chemical compounds, commonly known as secondary metabolites (Moore, Andrew, Külheim, & Foley, 2014) These are present mainly under stress conditions, but their absence does not cause harmful effects to the plants (Edreva, 2005; Edreva et al, 2008; Khan, Aliabbas, Kumar, & Rajkumar, 2009). Among them phenolic compounds are one of the most pronounced secondary metabolites found in plants, play an important role in plant interactions as defense chemicals and their distribution is shown throughout the entire metabolic process (Kurmukov, 2013). These can act as antioxidants, structural polymers (lignin), attractants for pollinators insects (flavonoids and carotenoids), UV screens (flavonoids), signal compounds (salicylic acid and flavonoids) and defense response chemicals (tannins and phytoalexins) (Brglez Mojzer, Knez Hrnčič, Škerget, Knez, & Bren, 2016). The aim of the present work was to develop a micropropagation protocol of B. antioquensis and evaluate the production of polyphenols by tissue culture after UV-B treatment
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