Abstract
Begonia fallax A.DC. belongs to the family of Begoniaceae and is an endemic species to Peninsular India. Shoot tip and nodal explants were screened using Murashige and Skoog medium supplemented with BAP, KIN and TDZ individually and combination with IAA, NAA and IBA for multiple shoot initiation. BAP and NAA (0.5+0.5 mg-l) produced 20.8 shoots with up to 4.0 cm shoot length to nodal explants whereas 17.9 shoots with 3.1 cm shoot length to shoot tip explants. For root induction, various concentrations of IAA, NAA and IBA were used individually. The highest rooting frequency was achieved to nodal explants (64.90 roots) on MS medium supplemented with 0.5 mg-l NAA compared to shoot tip explants (31.90 roots). Invariably, root length was up to 2.3 cm to both the explants. The rooted explants were transplanted into plastic cups and showed 70% survival rate in the Environmental Growth Chamber. Nodal explants could be the best for large-scale multiplication and propagation to facilitate in vitro conservation and for secondary metabolites production.
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