Abstract
Micropropagation studies were conducted to determine the best methods to achieve axillary shoot proliferation for adult Acer rubrum (red maple) and A. saccharium (sugar maple). The first experiment was conducted to compare the effects of 0.001, 0.01, 0.1 μM, 1 μM, and 10 μM thidiazuron (TDZ) using Driver-Kuniyuki-Walnut medium (DKW). The second experiment was conducted to examine the effects of DKW, Woody Plant Medium (WPM) and Long and Preece (LP) media in factorial combination with 0.01 and 0.1 μM TDZ. The third experiment was conducted to study the transfer timing (14 or 28 days) and the media solidification (agar-solidified or stationary liquid) on sugar maple. Both red maple and sugar maple explants on DKW with 0.1 μM TDZ produced the most and longest axillary shoots; however, sugar maple produced fewer axillary shoots than red maple. Red maple explants produced the most callus on DKW with 10 μM TDZ and the least on DKW with 0.001 μM TDZ. Sugar maple explants produced more shoots when explants were placed horizontally and transferred every 14 days than when placed vertically or transferred less frequently.
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