Abstract
A tissue culture protocol was developed for an important medicinal plant Rungia pectinata L. in the present study. Nodal shoots were used as explants and surface-sterilized with 0.1% HgCl2 solution. Murashige and Skoog (MS) medium was used to establish the cultures of R. pectinata. The bud break was reported on MS medium supplemented with 1.0 mg L−1 6-benzylaminopurine (BAP). About 98% response was observed with this media combination and maximum 3.2 shoots per explant with 4.3 cm length were recorded. The shoots were further multiplied using MS medium augmented with 0.5 mg L−1 each of BAP and kinetin (Kin) + 0.1 mg L−1 indole-3 acetic acid (IAA). Maximum 13.2 shoots per explant with 5.2 cm length were observed. All the shoots were rooted (4.9 roots per shoot with 3.5 cm length) on half strength MS medium fortified with 2.0 mg L−1 indole-3 butyric acid (IBA). In vitro flowering was induced from the shoots on half strength MS medium supplemented with same concentrations and combinations of growth regulators used for shoot multiplication under 12/12 hr light/dark photoperiod. The plantlets were hardened in the greenhouse for two months and finally transferred to the field. The foliar micromorphological studies revealed the developmental changes in stomata, vein density, and trichomes during the culture of shoots under in vitro conditions.
Highlights
Rungia pectinata L. is an ethnomedicinal plant which belongs to the family Acanthaceae
R. parviflora var. pectinata, R. parviflora var. muralis, and Justicia pectinata are the synonyms of this plant
Bud breaking from nodal meristems was observed in all the explants with all the concentrations of growth regulators within 7 days of inoculation, whereas no shoots were developed on Murashige and Skoog (MS) basal medium devoid of growth regulators
Summary
Rungia pectinata L. is an ethnomedicinal plant which belongs to the family Acanthaceae. Muralis, and Justicia pectinata are the synonyms of this plant. It is an annual erect herb with profuse branching and distributed throughout the warmer parts of India [1]. Phytosterols, terpenes, tannins, glycosides, flavonoids, phenolic compounds, amino acids, fixed oils, and so forth are important phytochemicals isolated from this plant [3]. The juice prepared from the leaves is considered as cooling agent and used to cure smallpox in the infants. The paste prepared from fresh leaves mixed with castor oil is reported to cure tinea capitis, a scaly fungoid infection on scalp. R. pectinata have been claimed to exhibit antipyretic, anti-inflammatory, diuretic, analgesic, antifungal, and antimicrobial activities [4, 8, 9]
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