Abstract
Rhizomes of juvenile sporophytes of Blechnum spicant L. and Pteris ensiformis L. cultured on MS medium with N6-benzyladenine alone (0.44–4.4 μM) or in combination with naphthaleneacetic acid (0.053–0.53 μM), respectively, gave rise to several proliferation centres located at the epidermal and inner parenchyma of this organ after one month in culture. Subculture of these rhizomes for one month in growth regulator-free medium allowed organization of internal proliferation centres, and regeneration of a large number of sporophytes. Subculture in the above-mentioned proliferation medium, induced phase change and development of aposporous gametophytes from surface-localized proliferation centres. Addition of naphthaleneacetic acid to the culture medium promoted proliferation of green globular bodies (GGB) in B. spicant and both rhizogenesis and callogenesis processes in P. ensiformis. GGB derived from rhizomes of B. spicant produced numerous sporophytes and from the point of view of micropropagation it is a good system to regenerate sporophytes in this species.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
