Abstract
Root tip explants from mature, blooming size Cypripedium explanted in Steele-modified Harvais medium containing potato extract were incubated with the plant growth regulators 2,4-dichlorophenoxyacetic acid (2,4-D) and N 6 -benzyladenine (BA) or kinetin but not 2,4-D or BA or kinetin alone, produced callus. If callus remained in contact with plant growth regulators multiple shoots and/or short roots developed. In contrast, if calli were reflasked into plant growth regulator-free media, then most calli produced plantlets that phenotypically resembled seed-derived seedlings. Plantlets resembling normal seedlings established readily following vernalization for 3-5 months whereas plantlets with multiple shoots and short roots grew more slowly with lower survival.
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