Abstract
A protocol for the genetic transformation and regeneration of Chinese elm ( Ulmus parvifolia Ja cq.) is reported. Cell suspension cultures were initiated from hypocotyls and were maintained on Murashige and Skoog (MS) basal semi-solid medium supplemented with varying concentrations of thiadiazuron (TDZ) and 1-naphthaleneacetic acid (NAA). Two week- old cell suspension cultures were bombarded with gold particles coated with plasmid harboring the Basta®resistance and â -glucuronidase genes. Elm cultures produced green calli within a month on selection media containing 20 mg L-1phosphinothrycin. These c alli were transferred to the same medium without the herbicide and were regenerated into shoots within 2 mo. The expression of uidA reporter gene in transformed elms was detected by histochemical analysis for â-glucuronidase activity. The presence of the Basta®resistance insert was demonstrated by polymerase chain reaction analysis. Key words:
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
