Abstract
A particle bombardment protocol using the Dupont Biolistic™ particle delivery system and the β-glucuronidase reporter gene was developed for embryogenic cell lines of Larix. Comparison of four sizes of tungsten microprojectiles showed the highest transient gene expression with 1.11 μm diameter particles. The highest β-glucuronidase transient gene activity was in cells bombarded 5 and 6 days after subculturing to fresh media. Comparison of 22 different cell lines of haploid and diploid Larixdecidua, Larixleptolepis, LarixXleptoeuropae, and LarixXeurolepis showed variation among the cell lines in β-glucuronidase gene activity, embryogenesis, and cell growth. In diploid lines, the levels of transient gene expression were higher with youngest lines; however, this trend was not distinguishable in haploid lines
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