Microplastics increase the accumulation of phenanthrene in the ovaries of marine medaka (Oryzias melastigma) and its transgenerational toxicity

Abstract

Microplastics (MPs) are considered to exacerbate the impacts of hydrophobic organic pollutants on aquatic organisms through the carrier function, but whether MPs affect the transgenerational toxicity of pollutants is unclear. This study exposed adult female marine medaka to phenanthrene (Phe)-adsorbed MPs for 60 days to investigate the effects of MPs on the bioaccumulation, reproductive and transgenerational toxicity of Phe. Compared to Phe alone, co-exposure to Phe and 200 μg/L MPs significantly increased Phe bioaccumulation in the intestines and ovaries. Phe alone and Phe combined with MPs disrupted the regulation of the hypothalamus-pituitary-gonadal axis, and reduced vitellogenin levels and reproductive capacity of female fish. In particular, co-exposure to 200 μg/L MPs and Phe increased the rate of follicular atresia, inhibited ovarian maturity, and aggravated reproductive toxicity. Notably, maternal uptake of Phe could be transferred to the offspring, and embryonic accumulation increased with the concentrations of MPs. Moreover, MPs aggravated Phe-induced bradycardia in embryos, suggesting that MPs exacerbated the transgenerational toxicity of Phe. These findings reveal that the growing number of MPs in the ocean might amplify the adverse effects of organic pollutants on the health and population stability of marine fishes, and this problem merits more attention.