Microplastics and their interaction with microorganisms in Bosten Lake water

Abstract

Microplastic pollution in inland freshwater lakes, formed from melting ice, atmospheric precipitation, and groundwater, remains under-researched. This study examines microplastic (MPS) presence and distribution in Bosten Lake and their interaction with microorganisms. We employed a Confocal Raman spectrometer to identify MPS types and 16S rRNA high-throughput sequencing to analyze microbial diversity and composition. In May, the lake's average microplastic concentration was 108.20 particles per liter (PCS/L), decreasing to 21.67 PCS/L by October. Transparent and yellow fibrous microplastics, predominantly 0.2–0.5 mm in size, were most common. Key microplastics in May included PEEK (27%), PMMA (25%), and PET (9%), while October saw a prevalence of PET (35%), PS (13%), and PA (9%). Higher microplastic concentrations correlated with reduced microbial diversity. In May, the high microplastic concentration group (MH) showed increased Planctomycetota, while in October, Patescibacteria and Bacteroidota rose significantly in the high plastic group (OH), known for microplastic degradation. MH also had a higher abundance of Cryptomycota compared to OH. Functional analysis indicated that MH had more genes related to bisphenol, benzene, and chlorine compounds, whereas OH had genes linked to methane, nitrogen, and organic matter degradation. Microplastic concentrations peaked near the lake inlet and tourist areas, affecting microbial quantity, diversity, structure, and function. This research enhances our understanding of MPS distribution and environmental microbial shifts in similar settings, providing essential data for environmental management strategies at Bosten Lake.