Abstract

Though the ultrafine short tips provided by our micropipette puller are helpful for many preparations, longer tips are still needed for some cases where target cells for intracellular work are deeply embedded in tissue. Because the original ‘loop’ type of heating filament proved unsuitable for widening to provide the longer tips, we studied simpler forms of heating filaments. By comparison with the common loop design, a rectangular trough filament proved to have many advantages without any significant disadvantage. In particular, this type of filament may be used at varying widths to provide long tips as well as short ones. Thus our micropipette puller can now provide tip lengths that extend upward by continuous gradations from about 6 to 27 mm, while maintaining tip diameter in the ultrafine range of 0.1 μm or less. These long tips have proved more reliable and free from bending than long tips traditionally provided by the Livingston puller. By using narrow filaments in conjunction with other parameters that influence tip size, tips have also been formed with diameters up to 1.5 μm and lengths of only 2 3 mm.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.