Abstract
A method for spectrophotometric determinations in the submicrogram range is described. By means of a shrinking droplet technique the optical density of small volumes was increased up to 300 times. The samples were shrunk in a Teflon cup by exposure to heated air. Measurements of the diameters of the small spheres formed, when oil was added, made it possible to calculate the volume reduction. The optical density of the sample was measured in a microscope photometer—either directly with the spherical droplets still remaining in the transparent Teflon cup or, with higher precision, after transfer of the sample to a hemocytometer cuvet sealed with oil.
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