Microphotometry of rat liver nuclear proteins. II. Microphotometry of rat liver nuclear proteins and RNA before and after partial hepatectomy.

Abstract

One hour after partial hepatectomy some liver nuclei already have an increased nuclear protein content, after 15 hours all measured nuclei, and after 64 hours only a proportion of the measured nuclei. A decrease of the Fast green histon content was found. This decrease occurred in the mayority of the nuclei 15 hours after partial hepatectomy. An increase in nuclear RNA was found for all measured nuclei at 15 hours, and for a proportion of the measured nuclei at 64 hours. Tetraploid and diploid nuclei behave identically. The excess of nuclear protein after hepatectomy is resistent to elution with 0.14 M NaCl, which indicates a binding with chromosomal proteins or DNA. The percentage of nuclear sap protein on the total nuclear protein content of tetraploid, diploid parenchymal nuclei and littoral cell nuclei, is equal. These nuclei differ in the amount of chromosomal bound proteins.