Abstract
Severe traction injuries after stretch to peripheral nerves are common and challenging to repair. The nerve guidance conduits (NGCs) are promising in the regeneration and functional recovery after nerve injuries. To enhance the repair of severe nerve traction injuries, in this study KHIFSDDSSE (KHI) peptides were grafted on a porous and micropatterned poly(D,L-lactide-co-caprolactone) (PLCL) film (MPLCL), which was further loaded with a nerve growth factor (NGF). The adhesion number of Schwann cells (SCs), ratio of length/width (L/W), and percentage of elongated SCs were significantly higher in the MPLCL-peptide group and MPLCL-peptide-NGF group compared with those in the PLCL group in vitro. The electromyography (EMG) and morphological changes of the nerve after severe traction injury were improved significantly in the MPLCL-peptide group and MPLCL-peptide-NGF group compared with those in the PLCL group in vivo. Hence, the NGCs featured with both bioactive factors (KHI peptides and NGF) and physical topography (parallelly linear micropatterns) have synergistic effect on nerve reinnervation after severe traction injuries.
Highlights
The peripheral nerve traction injury is one of the most common iatrogenic injuries in clinic (Schwartzman and Grothusen, 2008)
The guidance of directional migration of Schwann cells (SCs) is helpful to the functional recovery from severe damage in the peripheral nerve system, yet the application in practice still remains challenging despite of some pioneering studies (Zhang et al, 2018a)
nerve growth factor (NGF) can accelerate the process of nerve repair and regeneration, and its release plays a vital role for axon stretching
Summary
The peripheral nerve traction injury is one of the most common iatrogenic injuries in clinic (Schwartzman and Grothusen, 2008). The tractive damage on the nerve takes place mainly at the outer epineurium and perineurium, whereas the structure inside the endoneurium remains relatively intact (Chiang et al, 2008). These morphologic findings suggest that the nerve palsies from traction injuries are temporary, and the electromyography (EMG) would gradually gain partial recovery (Brauckhoff et al, 2018). The current standard of Conduits Synergistically Promote Nerve Repair nerve repair for severe traction injury is autografting (Zheng et al, 2018). There is a strong demand to find a new way for nerve repair after severe traction injury
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