Abstract
Abstract Previous short‐time experiments with Zea mays L. in nutrient solutions have shown that Se metabolism in plants depends on the oxidation state of the applied Se. Selenate was absorbed and translocated in xylem‐sap as selenate, while selenite was recognized in xylem‐sap as seleno amino acids a few minutes after application. A description is given of the methods used to separate the Se into fractions of selenate, selenite, seleno amino acids, and proteinbound Se. The methods are applied to barley grain and grass samples with and without pretreatment with pronase. The results show very little difference in the distribution of the total Se between these four fractions when the Se was applied as selenate or selenite and when it was sprayed on the plants or added to the fertilizers.
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