Abstract
Two known mutagenic drugs, cyclophosphamide and vinblastine sulfate, were tested using the micronucleus test in the native fish species, Astyanax bimaculatus, in order to determine which of these drugs and the doses which would be the most adequate for use as positive controls in this species. This Brazilian fish species was chosen because few toxicity studies have used native fish species and this particular species is widely consumed in various regions of Brazil. Three thousand erythrocytes per specimen were scored. Doses of 16 and 8 mg/kg body weight of cyclophosphamide and vinblastine sulfate, respectively, were the most effective in causing micronuclei. Cyclophosphamide was the most mutagenic of the two drugs and is recommended for use as a positive control in A. bimaculatus.
Highlights
Fish are excellent subjects for the study of the mutagenic and/or carcinogenic potential of contaminants present in water samples since they can metabolize, concentrate and store waterborne pollutants (Al-Sabti, 1991)
As a preliminary step in monitoring the levels of genotoxic pollutants in Brazilian rivers, we tested for the optimum dose of cyclophosphamide and vinblastine sulfate to use in native fish species
The number of cells analyzed was different for each group of the animals treated with mutagenic drugs because some fish died within 24 h after injection (Table I)
Summary
Fish are excellent subjects for the study of the mutagenic and/or carcinogenic potential of contaminants present in water samples since they can metabolize, concentrate and store waterborne pollutants (Al-Sabti, 1991). Chorvatovicová and Sandula (1995) recommended the use of this drug in chromosome aberration tests, sister chromatid exchanges and micronucleus (MN) formation in vitro and in vivo This drug, and vinblastine sulfate, an aneugenic agent, are mutagenic drugs usually used as positive controls in in vivo tests of short duration. The dose of cyclophosphamide and vinblastine sulfate used in routine mammalian test systems has been established, but this has not been done for native Brazilian fish Preliminary studies on this species treated with cyclophosphamide or vinblastine sulfate showed that the maximum response for micronucleus induction by these agents was 24 h after injection. As a preliminary step in monitoring the levels of genotoxic pollutants in Brazilian rivers, we tested for the optimum dose of cyclophosphamide and vinblastine sulfate to use in native fish species. In this study Astyanax bimaculatus was choosen because it is a common, small, detritivorous fish of considerable economic importance
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
