Abstract
Micronucleus Assay was employed to detect the effects of acute exposure of human peripheral blood lymphocytes in vitro to Cs -137 gamma rays. Human whole blood samples were irradiated with different doses of gamma rays namely ) 0.1, 0.2, 0.3, 0.4, 0.5, 1.00) Gy, respectively in addition to a control non-irradiated sample. The samples were tissue cultured and cytokinesis blocked method was used to investigate the frequency of micronuclei. In vitro exposure of lymphocytes to this doses led to elevation of micronuclei in comparison with non –irradiated samples However, inclusion of mono-, tri-,and quadrinucleated cells in micronucleus assay probably gives more satisfying result than restriction the test on binucleated cells. Computed programmed were employed to establish dose – response relationships to be used as biological dosimeter during radiation accidents.
Highlights
Gene expression using as Biomarkers for detection
A thesis submitted to the College of Science
عذول ( :)2يعذل ورىصَع انُىي انصغُشح فٍ انخلاَب انهًفبوَخ احبدَخ و صُبئُخ وصلاصُخ وسثبعُخ انُىاح انًعشضخ خبسط انغسى انحٍ
Summary
[9, 8زطيت اىزقٜٛط اىج٘ٞى٘ع ٜىلإشؼبع رؾؼٞش ٍْؾْٞبد قٞبعٞخ ٗػلاقبد سٝبػٞخ ر٘ػؼ اىؼلاقخ ث ِٞاىغشع الإشؼبػٞخ لأّ٘اع ٍخزيفخ ٍِ الأشؼخ اىَؤْٝخ ٍٗب ٝقبثيٖب ٍِ رأصٞشاد ف ٜاىَبدح اى٘ساصٞخ ٗٝزٌ رىل ثزؼشٝغ َّبرط ٍِ دً الإّغبُ خبسط اىغغٌ اىؾ In vitro ٜاى ٚعشع ٍزؼذدح ٍِ عُُبد انذو وطشائك انزشعُع عؾت (ٍ )20و ٍِ دً شخض ثؼَش ( )30عْخ ،لاٝذخِ ٗلاٝزؼبؽ ٚاىَششٗثبد اىنؾ٘ىٞخ ٗٗ ،ػغ اىذً اىَغؾ٘ة ف ٜاّبثٞت رؾ٘ ٛػيٍ ٚبّغ اىزخضش اىٖٞجبس Heparin ِٝثؼذ ػَيٞخ اىغؾت ٍجبششح ٗ .ثؼذٕب رٌ ر٘صٝغ ػْٞبد اىذً اىَغؾ٘ثخ فٍ ٜؾبقِ ؽجٞخ ثلاعزٞنٞخ ٍؼقَخ عؼخ ( )1عٌ 3ثَؼذه ( )0.5عٌ 3ف ٜمو ٍؾقْخ ؽجٞخ ثلاعزٞنٞخ ٍِٗ صٌ اعشٝذ ػَيٞخ رشؼٞغ ٕزٓ اىَْبرط ٗرىل ثزؼشٝؼٖب اى ٚعشع إشؼبػٞخ ٕ1.0, 0.5,0.4, ( ٜ )0.3, 0.2 ,0.1,0.0غشا ٍِ ٛأشؼخ مبٍب اىظبدسح ٍِ ػْظش اىغٞض ً٘ٝــ 137ثأعزخذاً عٖبص Gamma cell – 40اىَظْغ ٍِ قجو ششمخ ٗ imited ،Atomic Energy of Canada lاُ اىَظذس اىَغزخذً ٝؼطٜ عشػخ اشؼبػٞخ ٍقذاسٕب ( )64.23غشا / ٛعبػخ ٗ ،ثؼذ اّزٖبء ػَيٞخ رشؼٞغ اىَْبرط ؽؼْذ ثذسعخ ؽشاسح 37
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